Journal: iScience

Article Title: Human SKI component SKIV2L regulates telomeric DNA-RNA hybrids and prevents telomere fragility

doi: 10.1016/j.isci.2024.111096

Figure Lengend Snippet: SKIV2L and TTC37 prevents telomere fragility (A and B) Telomere FISH analysis in SKIV2L- and TTC37-depleted HeLa1.3 and HT1080-ST cells using siRNAs: % of telomere fragility (yellow) and loss (purple) per metaphase in siCtr, siSKIV2L and siTTC37 (means ± SD, n > 25 metaphases, 2 independent experiments, scale bar, 10 μm). t test ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001. (C) Telomere FISH analysis in shCtr (Control) or shSKIV2L HEK293 cells expressing GFP or SKIV2L (means ± SD, n > 25 metaphases, 2 independent experiments, scale bar, 10 μm). Details are as in (A). (D) Telomere FISH analysis in TTC37-depleted HEK293 cells using siRNAs (means ± SD, n = 25 metaphases). Details are as in (A). (E) Telomere FISH analysis in HeLa1.3 cells using shRNAs: shCtr and shSKIV2L treated with DMSO (−, control) or APH (+) ( n > 45 metaphases, 2 independent experiments). Details are as in (A). (F) IF of pre-extracted cells showing co-localization of pS1981 ATM autophosphorylation with TRF2 (telomeres) in shCtr and SKIV2L-depleted (shSKIV2L) HeLa1.3 cells. Quantification of the percentage of cells with co-localization foci and mean number of foci per nucleus is depicted (means ± SEM, n = 200 cells, 2 independent experiments, scale bar 15 μm). t test ∗∗∗ p < 0.001. (G) IF-FISH of pre-extracted cells showing co-localization of 53BP1 with telomeres in asynchronous (AS) or G2-synchronized control (shCtr) and SKIV2L-depleted (shSKIV2L) HeLa1.3 cells. Quantification of the percentage of cells with co-localization foci is depicted (means ± SEM, n > 400 cells, 3 independent experiments, scale bar 15 μm). t test ∗ p < 0.05. See also Figure S2 .

Article Snippet: Rabbit polyclonal anti-53BP1 , Novus Biologicals , Cat#NB 100-304, RRID: AB_350221.

Techniques: Control, Expressing

Journal: iScience

Article Title: Human SKI component SKIV2L regulates telomeric DNA-RNA hybrids and prevents telomere fragility

doi: 10.1016/j.isci.2024.111096

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-53BP1 , Novus Biologicals , Cat#NB 100-304, RRID: AB_350221.

Techniques: Virus, Recombinant, Protease Inhibitor, Reverse Transcription, Blocking Assay, Mass Spectrometry, SYBR Green Assay, Flow Cytometry, Imaging, Mutagenesis, Cell Cycle Assay, shRNA, Software

Journal: Journal of Radiation Research

Article Title: Detection of genome instability by 53BP1 expression as a long-lasting health effect in human epidermis surrounding radiation-induced skin cancers

doi: 10.1093/jrr/rrae035

Figure Lengend Snippet: Types of TP53-binding protein 1 expression (green) by dual-color immunofluorescent analysis with Ki-67 expression (red). (A and F) Stable DDR type, no or faint nuclear staining. (B and G) Low DDR type, one or two discrete NF. (C and H) High-DDR type, three or more discrete NF. (D and I) Diffuse type, intense heterogeneous nuclear staining. (E and J) Large NF type, discrete NF >1.0 μm. The incidence of high DDR, large foci or diffuse types in the nuclei is considered as abnormal 53BP1 expression. Scale bars indicate 2 μm.

Article Snippet: For dual-color IF, the sections were incubated with anti-53BP1 rabbit polyclonal antibody (1:1000; A200-272A; Bethyl Laboratories, Montgomery, TX) and anti-Ki-67 mouse antibody (1:50; MIB-1; DakoCytomation, Glostrup, Denmark) for 1 h at 20°C.

Techniques: Binding Assay, Expressing, Staining

Journal: Journal of Radiation Research

Article Title: Detection of genome instability by 53BP1 expression as a long-lasting health effect in human epidermis surrounding radiation-induced skin cancers

doi: 10.1093/jrr/rrae035

Figure Lengend Snippet: Incidence of types of 53BP1 expression in epidermis by IF

Article Snippet: For dual-color IF, the sections were incubated with anti-53BP1 rabbit polyclonal antibody (1:1000; A200-272A; Bethyl Laboratories, Montgomery, TX) and anti-Ki-67 mouse antibody (1:50; MIB-1; DakoCytomation, Glostrup, Denmark) for 1 h at 20°C.

Techniques: Expressing, Irradiation

Journal: Journal of Radiation Research

Article Title: Detection of genome instability by 53BP1 expression as a long-lasting health effect in human epidermis surrounding radiation-induced skin cancers

doi: 10.1093/jrr/rrae035

Figure Lengend Snippet: Dual-labeled IF of TP53-binding protein 1 (green) and γH2AX (red) expression in cancer cells of patients with BD. Scale bars indicate 20 μm. 53BP1, TP53-binding protein 1.

Article Snippet: For dual-color IF, the sections were incubated with anti-53BP1 rabbit polyclonal antibody (1:1000; A200-272A; Bethyl Laboratories, Montgomery, TX) and anti-Ki-67 mouse antibody (1:50; MIB-1; DakoCytomation, Glostrup, Denmark) for 1 h at 20°C.

Techniques: Labeling, Binding Assay, Expressing